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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and therefore may possibly contribute to tumorigenesis. This protein was observed to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant purpose in selling cell-cycle development within the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, have already been explained. Two supplemental splice variants were being described but detailed nucleotide sequence information and facts is just not however available.
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Blocking peptides are peptides that bind particularly to your target antibody and block antibody binding. These peptide commonly incorporates the epitope recognized because of the antibody.
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This guide outlines further possible optimization of mobile and immunoassay parameters to be sure the absolute best results are...
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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to be sure the absolute best benefits are...
Antibodies sure to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.
Goal Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterised by a spectacular periodicity in protein abundance with the cell cycle. Cyclins functionality as regulators of CDK kinases. Unique cyclins exhibit distinct expression and degradation styles which lead to the temporal coordination of each and every mitotic function. Cyclin E types a complex with and features being here a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells progress by S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both antibodies...
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